VIABILITY AND PLASMA MEMBRANE INTEGRITY OF THE SPOTTED BUFFALO EPIDIDYMAL SPERMATOZOA AFTER THAWING WITH THE ADDITION OF DEXTROSE INTO THE EXTENDER

Th e objective of this study was to obtain the viability and plasma membrane integrity of the spotted buff alo epididymal sperm after addition of dextrose into Andromed ® extender. Spermatozoa that have been collected from cauda epididymis were diluted with Andromed ® extender as control (K) and Andromed ® + 0.2 % dextrose (P1) and Andromed ® + 0.4 % dextrose (P2) as treatments. Th e results showed that the quality of epididymal spermatozoa decreased during cryopreservation process. Th e percentage of motility after thawing in P1 (46%) and P2 (46.67%) were signifi cantly higher (P<0.05) compared to K (41%) as well as the percentage of live sperm in P1 (58.8%) and P2 (60%) compared to K (52.2%). Th e percentage of membrane integrity in P1, P2 and K were 67.4; 66.8 and 68 %, respectively. In conclusion, the addition of 0.2 and 0.4 % of dextrose into Andromed ® acted as an extra cellular cryoprotectant and could maintain the viability and membrane integrity of the spotted buff alo epididymal spermatozoa after thawing. Key words: epididymal sperm, cryopreservation, dextrose, spotted buffal