East Java, Indonesia

In the microbial world, pigments are one of the most conspicuous traits. Marine bacteria associated with sp. collected from Taka Cemara, Karimunjawa Islands were screened for the production of a yellow pigment. The isolation of bacterial symbionts from sp. on Zobell 2216E medium resulted in one bacterium, KJ5, positively synthesized carotenoids. By reverse phase HPLC analysis, one peak of the pigment types was identified as a peak which appeared at 60.24 min. Then, sample of the was collected and identified according to their spectral characteristics and compared with the published data in different types of solvent. Based on the HPLC analysis, the total carotene contents were calculated by converting the broad absorption of carotene. Molecular identification of the bacterium KJ5 using 16S rDNA showed that bacterium KJ5 was closely related to with 96% homology value., sp., , , HPLC akteri laut berwarna kuning telah berhasil diisolasi dari karang lunak yang berasal dari Taka Cemara, Karimunjawa, Jawa Tengah. Dari hasil skrining pada media ZoBell 2216E ditemukan satu isolat KJ5 yang diduga mengandung karotenoid. Identifikasi karoten dengan fase terbalik kromatografi cair kinerja tinggi (KCKT) berhasil mengidentifikasi-karoten yang muncul pada menit ke 60,24.-karoten murni diperoleh dengan menampung hasil KCKT yang selanjutnya dianalisa dengan UV-Tampak. Spektra diidentifikasi sesuai dengan karakteristiknya dan dibandingkan dengan referensi lain dalam berbagai jenis pelarut. Identifikasi spesies bakteri yang dilakukan menggunakan reaksi berantai polimerase, menunjukkan bahwa isolat bakteriKJ5memiliki homologi sebesar 96%dengan Kata kunci: sp., , HPLC, 16SRDNA β-carotene β-carotene β-β-β-carotene keberadaan β β β-karoten