Metabolic Properties of Mouse Transplant- able Adenocarcinoma. II. Substrate Utiliza- tion by Homogenates 1,2

SUMMARY-The oxidation of some Krebs cycle substrates by homogenates of submaxillary gland adenocarcinoma and of normal submaxillary gland and the eFfect of diphosphopyridine nucleotide (DPN) on the oxygen consumption in C3H mice were investigated. Homogenates of normal tissue had a higher basal rate of oxygen consumption, but the percentual increases after substrates were added were smaller than those in homoge-nates of tumors. Adding DPN not only raised the basal oxidation rate of the tumor homogenates to values similar to normal tissue, but also raised the percentual increases. The extent of the increase in the tumor homogenates was also greater than that of the increase in the normal submaxillary gland. However, the total rates of oxygen consumption (with or without added substrates andlor DPN) were usually higher in the normal tissue homogenates than in tumor homogenates.-J Nat Cancer Inst 47: 271-275,1971. THE SUBMAXILLARY gland adenocarcinoma of C3H mice differs metabolically from the norma