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Heat shock proteins (HSPs) are proteins that are synthesized by Laemmli [1]. Aliquots corresponding to 12.5 mg of protein were applied to each of the wells. A low-molecular-weight protein stan-prokaryotic as well as eukaryotic cells when exposed to elevated temperatures or to a variety of other stresses. These proteins enable dard (SDS-PAGE Standards, Bio-Rad) was included. The gels were transblotted to nitrocellulose membranes, and thethe cells to survive under unfavorable conditions and also appear to be essential for cell survival in normal situations. HSPs are electroblots were exposed to Kodak XAR-5 film (Kodak; Roches-ter, NY) for 5–10 days at 0707C. Blots that gave weak signalshighly evolved conserved molecules that are classified into families according to molecular weights: HSP90, HSP70, HSP60, and small were exposed for another 10 days. After development, the auto-radiographs were scanned, and the bands were analyzed by meansHSPs. Figure 1. De novo protein synthesis in Bacte-roides fragilis, strains 30285 and NCTC (Nationa