Two-DimensionalGel Electrophoresisof CerebrospinalFluidProteins’

Two-dimensional electrophoresis, with isoelectric focusing in the first dimension and sodium dodecyl sulfate/poly-acrylamide gel electrophoresis in the second, has been adapted for the high-resolution analysis of cerebrospinal fluid proteins. Proteins were detected with a new, highly sensitive silver stain that made visible more than 300 polypeptides from 60 j.tL of spinal fluid, in highly repro-ducible patterns. We have mapped these patterns, noting differences between the proteins observed in spinal fluid and plasma, and have prepared a partial map of cerebro-spinal fluid proteins. AddItional Keyphrases: peptides “mapping ” of CSF proteins. comparison with plasma A technique allowing simultaneous observation of many different proteins would be of obvious utility in searching fo