Add to ORKGThe use of autonomous replicons derived from plasmids and bacteriophages in the construction of cloning vectors for use in prokaryotic cells has been essential to the revolution in biological knowledge which has occurred since the advent of recombinant DNA technology. A large variety of such prokaryotic vectors, many of them tailored to particular experimenta
I describe the construction of a variety of Escherichia coli recA deletion strains designed to facil...
We have constructed a number of plasmids selectable in both E. coli and mouse or human cells. Human ...
We describe the construction and use of two classes of cDNA cloning vectors. The first class compris...
The use of autonomous replicons derived from plasmids and bacteriophages in the construction of clon...
Use of Escherichia coli bacteria as a host for high-level expression of cloned genes has become comm...
A multipurpose vector vas constructed which can be used for cloning DNA fragments of about 20 kb gen...
The emergence of recombinant DNA technology during the early 70's set a revolution in molecular biol...
DNA cloning vectors were developed which utilize the replication origin (ori) of bacteriophage fd fo...
Biochemical, biophysical and genetic studies of DNA segments of complex genomes are greatly facilita...
International audiencePlasmids play a central role in engineering recombinant bacteria because they ...
"High yields of specific genes or their products can be obtained by cloning the genes (from any orga...
The method uses a novel plasmid vector, p9lox5, containing a site-specific recombination sequence lo...
Most conditional expression vectors designed for mammalian cells have been valuable systems for stud...
The aim of this work was to develop a more efficient and usable basic form of cloning vehicle for ge...
We seek to create useful biological diversity by exploiting the modular nature of genetic informa-ti...
I describe the construction of a variety of Escherichia coli recA deletion strains designed to facil...
We have constructed a number of plasmids selectable in both E. coli and mouse or human cells. Human ...
We describe the construction and use of two classes of cDNA cloning vectors. The first class compris...
The use of autonomous replicons derived from plasmids and bacteriophages in the construction of clon...
Use of Escherichia coli bacteria as a host for high-level expression of cloned genes has become comm...
A multipurpose vector vas constructed which can be used for cloning DNA fragments of about 20 kb gen...
The emergence of recombinant DNA technology during the early 70's set a revolution in molecular biol...
DNA cloning vectors were developed which utilize the replication origin (ori) of bacteriophage fd fo...
Biochemical, biophysical and genetic studies of DNA segments of complex genomes are greatly facilita...
International audiencePlasmids play a central role in engineering recombinant bacteria because they ...
"High yields of specific genes or their products can be obtained by cloning the genes (from any orga...
The method uses a novel plasmid vector, p9lox5, containing a site-specific recombination sequence lo...
Most conditional expression vectors designed for mammalian cells have been valuable systems for stud...
The aim of this work was to develop a more efficient and usable basic form of cloning vehicle for ge...
We seek to create useful biological diversity by exploiting the modular nature of genetic informa-ti...
I describe the construction of a variety of Escherichia coli recA deletion strains designed to facil...
We have constructed a number of plasmids selectable in both E. coli and mouse or human cells. Human ...
We describe the construction and use of two classes of cDNA cloning vectors. The first class compris...