Identification of proteomic biomarkers in maternal plasma in the early second trimester that predict the subsequent development of gestational diabetes

Introduction: This study is designed to identify proteomic biomarkers that predict the subsequent development of gestational diabetes mellitus (GDM). Methods: Maternal blood was obtained prospectively from healthy pregnant women in the early second trimester (16-20 weeks). Twelve women subsequently diagnosed with GDM at 24 to 28 weeks were selected as cases; an equal number of normoglycemic women as controls. Proteomic analysis of the previously stored plasma was performed by surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry. Results: Three peaks (9122 Da, 9412 Da