During nucleosome assembly, newly synthesized H4 is acetylated prior to its d...

ABSTRACT 16 The Hat1 histone acetyltransferase has been implicated in the acetylation of histone 17 H4 during chromatin assembly. In the following report we have characterized the Hat1 18 complex from the fission yeast Schizosaccharomyces pombe, and have examined its role in 19 telomeric silencing. Hat1 is found associated with the RbAp46 homologue Mis16, an 20 essential protein. The Hat1 complex acetylates lysines 5 and 12 of histone H4, the sites that 21 are acetylated in newly synthesized H4 in a wide range of eukaryotes. Deletion of hat1 in S. 22 pombe is itself sufficient to cause the loss of silencing at telomeres. This is in contrast to 23 Copyright © 2012, American Society for Microbiology. All Rights Reserved. Eukaryotic Cell doi:10.1128/EC.00123-12 EC Accepts, published online ahead of print on 6 July 2012 Hat1, H4 and telomeric silencing in fission yeast 2 results obtained with an S. cerevisiae hat1∆strain, which must also carry mutations of 24 specific acetylatable lysines in the H3 tail domain for loss of telomeric silencing to occur. 2