threatened Bromeliad plant which grows in the states of

The present research aimed to develop a reliable technique for in vitro conservation of Aechmea bambusoides germplasm. A. bambusoides germplasm was successfully established in vitro through seeds obtained from field collection. The plant multiplication was achieved in a liquid Murashige and Skoog medium without growth regulators and its long term culture maintained at least for 1.5 year without losing their growth ability. Axillary shoots obtained from A. bambusoides in vitro grown plants were readily acclimatized in a substrate containing either Dicksonia sellowiana fiber (xaxim) or Eichhornia crassipes fiber (aguapé). Key-words: in vitro establishment, germplasm conservation, bromeliads, acclimatization, Dicksonia sellowiana fiber, Eichhornia crassipes fiber RESUMO Conservação in vitro de Aechmea bambusoides L.B. Smith & Reitz, uma espécie de bromélia brasileira ameaçada de extinção O objetivo do presente trabalho foi desenvolver uma técnica segura para conservação in vitro do germoplasma de Aechmea bambusoides. O germoplasma de Aechmea bambusoides foi estabelecido in vitro através de semente