The effects of three extenders on refrigerated boar semen

This study aims to evaluate the quality of boar sperm that was refrigerated for 14 days at 17 ºC with three extenders. For this study, samples from four boars were collected twice a week using the gloved-hand technique. After collection, only ejaculates showing total motility of 75 % or greater were submitted to the refrigeration process. Ejaculates were diluted in Androhep, MR-A ® or Reading. Each portion was kept for two hours at 22 ºC, then sperm motility was assessed through contrast microscopy. Sperm mitochondrial activity, viability and acrosome integrity were measured by the flow cytometric technique. The remaining diluted semen was maintained at 17 ºC for 336 h, and the same analysis being repeated every 48 h. Semen diluted in Androhep revealed no significant quality deterioration in percentage of live spermatozoa during refrigeration. However, after 144 h, viability decreased significantly for MR-A ® and Reading (63.3 % ± 7.0 and 66.4 % ± 6.2, respectively), and after 336 h, this decrease was accentuated (56.3 % ± 3.9 and 18.4 % ± 6.2, respectively, for MR-A ® and Reading). On average, for all three extenders, acrosome integrity values did not differ statistically up to 144 h, ranging from 48.3 ± 2.3 for MR-A ® to 62.4 ± 3.2 for Androhep. Then values decreased towards the end of the experiment, with Androhep always presented the higher values