P189 SMOKING STATUS PREDICTS BENEFIT FROM BREATHING

smoking habit. A number of point-of-care urine cotinine tests are used to validate self-reported smoking and in some instances, provide feedback to improve smoking cessation. Urine testing is inappropriate in some instances, and while saliva testing is more acceptable, it is more difficult because cotinine is in lower concen-trations compared to urine. A prototype saliva test was developed and evaluated, but the colorimetric assay was deemed inadequate. A new, more sensitive assay has been developed and evaluated in a group of healthy volunteers. Method Volunteers (n117), aged between 22 and 67 years (36% female), including 61 smokers with a cigarette consumption of five or more cigarettes/day, (mean 16.0), provided a saliva sample using a manufactured collecting device. One ml of saliva was eluted using the test’s fixed-volume syringe. The sample was introduced onto freeze-dried reagents and quickly shaken. A sample positive for nico