c © Asociación Ibérica de Limnología, Madrid. Spain. ISSN: 0213-8409 Freshwater food web studies: a plea for multiple tracer approach

Freshwater food web studies: a plea for multiple tracer approach Food webs are complex systems of interactions between ecosystem species. Beyond the direct analysis of stomach contents, stable isotopes of carbon (δ13C) and nitrogen (δ15N) have been used widely to evaluate these trophic relationships and calculate the relative contribution of food sources to a consumer’s diet using mixing models. However, there are still some constraints on the use of these traditional tracers that limit their output. Here, we briefly comment on the potential of using multiple tracers (i.e., stable isotopes of C, N and H; trace metals), and applying recent numerical approaches (i.e. Bayesian mixing models) to advance the understanding of complex aquatic food webs. Stable isotopes of hydrogen (δ2H), normally used to examine large-scale migration patterns of terrestrial animals, have been recently proposed as a complementary trophic tracer in aquatic ecosystems. The principle for this application is the large isotopic difference in δ2H among food items that can be found in some aquatic systems. Other potential trophic indicators are such substances that accumulate through diet (e.g., trace metals). These substances are traditionally studied from bioaccumulation or toxicological perspectives, but there are indications that encourage their use for tracing food web interactions. Bayesian mixing models, which are able to incorporate several sources of variability and multiple food sources in the model, can help to solve puzzling results. In summary, we suggest that the simultaneous use of multiple tracers will provide more reliable results than any of the