Localization of the corticotropin-releasing hormone receptor gene on mouse

90 progeny from an interspecific backcross, (C57BL/6J • M. spre-tus)F 1 • C57BL/6J [1]. Molecular reagents: A 389-bp rat Crhr cDNA (rCRH-R7) con-taining coding sequence corresponding toamino acids 203 through 332 of the corticotropin-releasing hormone (CRH) receptor was produced by RT-PCR amplification of polyadenylated rat cortex RNA. The oligonucleotides u ed for the PCR amplification were: 5'-ATGTT C/T TT C/T TGGATGTT C/T GG- 3 ' and 5'- AC G/A AA G/A AANA G/A CAT G/A TANGT-3'. These degenerate primers were designed on the basis of the human CRH receptor amino acid sequence [2]. The identity of the clone was confirmed by comparison of the partial DNA sequence of rCRH-R7 to the rat CRH receptor sequence [3,4]. Allele detection: A BglI polymorphism was detected in mouse genomic DNA by hybridization of Southern blots with the rCRH