Transcription factor TFIIIB and transcription by RNA polymerase III

pol (RNA polymerase) III is charged with the task of transcribing nuclear genes encoding diverse small structural and catalytic RNAs. We present a brief review of the current understanding of several aspects of the pol III transcription apparatus. The focus is on yeast and, more specifically, on Saccharomyces cerevisiae; preponderant attention is given to the TFs (transcription initiation factors) and especially to TFIIIB, which is the core pol III initiation factor by virtue of its role in recruiting pol III to the transcriptional start site and its essential roles in forming the transcription-ready open promoter complex. Certain relatively recent developments are also selected for brief comment: (i) the genome-wide analysis of occupancy of pol III-transcribed genes (and other loci) by the transcription apparatus and the location of pol III transcription in the cell; (ii) progress toward a mechanistic and molecular understanding of the regulation of transcription by pol III in yeast; and (iii) recent experiments identifying a high mobility group protein as a fidelity factor that assures selection of the precise transcriptional start site at certain pol III promoters. The pol (RNA polymerase) III transcription apparatus is devoted to the production of small structural and catalytic RNAs. For the budding yeast Saccharomyces cerevisiae (Sc), it is fully enumerated; for the human, some gaps remain in the specification of the component parts and understanding their functions. This brief account emphasizes the current understanding of transcription by pol III in budding yeast and only briefly comments on the divergences of other pol III systems (see also [1–5]). The Supplementary Sectio