Equine thrombospondin II and secreted protein acidic and cysteine-rich in a model of normal and pathological wound repair

Equine thrombospondin II and secreted protein acidic and cysteine-rich in a model of normal and pathological wound repair. Physiol Genomics 38: 149–157, 2009. First published April 28, 2009; doi:10.1152/physiolgenomics.90383.2008.—Wound healing in horses is complicated, particularly when wounds are on the limb. The objectives of this study were to clone equine thrombospondin II (THBS2) and secreted protein acidic and cysteine-rich (SPARC) cDNAs and to compare the spatiotemporal expression of mRNAs and proteins during repair of body and limb wounds. These molecules were targeted in view of their potential biological contribution to angiogenesis, which is exacerbated during the repair of limb wounds in horses. Cloning was achieved by screening size-selected cDNA libraries previously derived from 7-day-old wounds. Expression was studied in unwounded skin and in samples from 1, 2, 3, 4, and 6 wk old wounds of the body and limb. Temporal gene expression wa