MM: Mutations in the alpha subunit of RNA polymerase that affect the regulation of porin gene transcription in Escherichia coli K-12

The two-component regulatory system consisting of OmpR and EnvZ controls the differential expression of major outer membrane porin proteins OmpF and OmpC of Escherichia coli K-12. We have isolated and characterized two mutations in rpoA, the gene encoding the alpha subunit of RNA polymerase, that decrease the expression of OmpF. These mutations have a number of properties that distinguish them from previously isolated rpoA mutations that affect porin expression. The rpoA203 mutation decreases the expression of porin genes ompF and ompC and also decreases the expression of the malE and phoA genes. In contrast, rpoA207 decreases the expression of ompF but does not affect ompC, malE, orphoA transcription. Our results suggest that mutations at various positions in the alpha subunit may affect the OmpR-dependent transcription ofompF and ompC differently and may be useful for analyzing the mechanism underlying their differential expression in response to medium osmolarity. An important question regarding the regulation of gene expression is how sets of genes are induced in response to an external or internal signal. In prokaryotes, many of these responses are mediated by pairs of regulatory proteins that belong to a large family of homologous proteins, known a