Various methods with slight variations in protocol exist for establishing and expanding neurosphere cultures. Generally, neurospheres are derived from a single-cell suspension of neural stem and progenitor cells isolated from the adult or fetal central nervous system (CNS), but neurosphere cultures can also be established from ES cells (3). The starting pop-ulation of cells is usually plated as a single-cell suspension on uncoated plastic in a N2 and/o
Stem cell-based neuronal differentiation has provided a unique opportunity for disease modeling and ...
AbstractStem cell-based neuronal differentiation has provided a unique opportunity for disease model...
Neural precursor cells (NPCs) with high proliferative potential are commonly expanded in vitro as ne...
After the initial reports of free-floating cultures of neural stem cells termed neurospheres (1,2), ...
Murine embryonic day 14 or E14 neural stem cells (NSCs), first isolated and characterized as a stem ...
This culture system, developed for the induction and selective amplification of neural stem/progenit...
In the last decade renewed enthusiasm in the neuroscience field has been sparked by the discovery of...
For most of the past century, the prospect of replacing lost or damaged cells in the central nervous...
The neurosphere assay is the most widely used in vitro tool to determine the proliferative and diffe...
Neural stem cells (NSCs) are important multipotent adult stem cells capable of self-renewal and diff...
<p><strong>INTRODUCTION </strong>A widely used in vitro culture, the neurosphere assay (NSA) has pro...
The neurosphere assay and the adherent monolayer culture system are valuable tools to determine the ...
The neurosphere assay is the standard retrospective assay to test the self-renewal capability and mu...
Since their initial description in 1992, neurospheres have appeared in some aspect of more than a th...
<p>(A) Bright field image of neurosphere derived from human postnatal gut cultured for 2 weeks. Neur...
Stem cell-based neuronal differentiation has provided a unique opportunity for disease modeling and ...
AbstractStem cell-based neuronal differentiation has provided a unique opportunity for disease model...
Neural precursor cells (NPCs) with high proliferative potential are commonly expanded in vitro as ne...
After the initial reports of free-floating cultures of neural stem cells termed neurospheres (1,2), ...
Murine embryonic day 14 or E14 neural stem cells (NSCs), first isolated and characterized as a stem ...
This culture system, developed for the induction and selective amplification of neural stem/progenit...
In the last decade renewed enthusiasm in the neuroscience field has been sparked by the discovery of...
For most of the past century, the prospect of replacing lost or damaged cells in the central nervous...
The neurosphere assay is the most widely used in vitro tool to determine the proliferative and diffe...
Neural stem cells (NSCs) are important multipotent adult stem cells capable of self-renewal and diff...
<p><strong>INTRODUCTION </strong>A widely used in vitro culture, the neurosphere assay (NSA) has pro...
The neurosphere assay and the adherent monolayer culture system are valuable tools to determine the ...
The neurosphere assay is the standard retrospective assay to test the self-renewal capability and mu...
Since their initial description in 1992, neurospheres have appeared in some aspect of more than a th...
<p>(A) Bright field image of neurosphere derived from human postnatal gut cultured for 2 weeks. Neur...
Stem cell-based neuronal differentiation has provided a unique opportunity for disease modeling and ...
AbstractStem cell-based neuronal differentiation has provided a unique opportunity for disease model...
Neural precursor cells (NPCs) with high proliferative potential are commonly expanded in vitro as ne...