Add to ORKGBACKGROUND: Measurement of serum androgens is im-portant in adult, geriatric, pediatric endocrinology, and oncology patients. We developed a liquid chroma-tography—tandem mass spectrometry (LC-MS/MS) assay for simultaneous measurement of androstenedi-one, dehydroepiandrosterone (DHEA), and testoster-one in these patients. METHODS: We spiked 200 L of serum or plasma with isotope-labeled internal standards and performed ex-traction withmethyl t-butyl ether.We then derivatized the extracts with hydroxylamine and analyzed them by LC-MS/MS using a 2-dimensional chromatographic separation with a 3.5-min analysis time. RESULTS: Total imprecision for each analyte was 11.2%. Limits of quantification were 10, 50, and 1
Background: The simultaneous quantification of a steroid hormones panel provides more clinical infor...
experience in developing liquid chromatography–tandem mass spectrometry (LC-MS/MS)1meth-ods for rout...
Steroid measurement is a challenge in pediatric endocrinology. Currently, liquid chromatography with...
BACKGROUND: Measurement of serum androgens is im-portant in adult, geriatric, pediatric endocrinolog...
thesisDehydroepiandrosterone and androstenedione are precursors of sex hormones; testosterone is a b...
Objectives: To develop a robust liquid chromatography-tandem mass spectrometry (LC–MS/MS) method to ...
problems with androgen immunoassays compared with mass spectrometry analyses. METHODS: We developed ...
11-Oxygenated androgens (11-OAs) are being discussed as potential biomarkers in diagnosis and therap...
Steroid analysis in clinical laboratories is dominated by immunoassays (IAs) that have a high sample...
The simultaneous, rapid and reliable measurement of a wide steroid panel is a powerful tool to unrav...
problems with androgen immunoassays compared with mass spectrometry analyses. METHODS: We developed ...
BACKGROUND: Liquid-chromatography tandem mass spectrometry (LC-MS/MS) has become the method of choic...
A liquid chromatography–tandem mass spectrometry (LC-MS/MS) method is described that employs a novel...
The physiological and pathological development of the breast is strongly affected by the hormonal mi...
peer reviewedBACKGROUND: Androstanediol glucuronide is linked to a range of disorders of peripheral ...
Background: The simultaneous quantification of a steroid hormones panel provides more clinical infor...
experience in developing liquid chromatography–tandem mass spectrometry (LC-MS/MS)1meth-ods for rout...
Steroid measurement is a challenge in pediatric endocrinology. Currently, liquid chromatography with...
BACKGROUND: Measurement of serum androgens is im-portant in adult, geriatric, pediatric endocrinolog...
thesisDehydroepiandrosterone and androstenedione are precursors of sex hormones; testosterone is a b...
Objectives: To develop a robust liquid chromatography-tandem mass spectrometry (LC–MS/MS) method to ...
problems with androgen immunoassays compared with mass spectrometry analyses. METHODS: We developed ...
11-Oxygenated androgens (11-OAs) are being discussed as potential biomarkers in diagnosis and therap...
Steroid analysis in clinical laboratories is dominated by immunoassays (IAs) that have a high sample...
The simultaneous, rapid and reliable measurement of a wide steroid panel is a powerful tool to unrav...
problems with androgen immunoassays compared with mass spectrometry analyses. METHODS: We developed ...
BACKGROUND: Liquid-chromatography tandem mass spectrometry (LC-MS/MS) has become the method of choic...
A liquid chromatography–tandem mass spectrometry (LC-MS/MS) method is described that employs a novel...
The physiological and pathological development of the breast is strongly affected by the hormonal mi...
peer reviewedBACKGROUND: Androstanediol glucuronide is linked to a range of disorders of peripheral ...
Background: The simultaneous quantification of a steroid hormones panel provides more clinical infor...
experience in developing liquid chromatography–tandem mass spectrometry (LC-MS/MS)1meth-ods for rout...
Steroid measurement is a challenge in pediatric endocrinology. Currently, liquid chromatography with...