ElectrophoreticSeparationof High-DensityLipoproteinCholesterol Evaluated and Compared with the Modified LipidResearch Clinic Procedure

We evaluated a new agarose-gel-electrophoretic proce-dure (Corning) (I) for separatingandquantitating of high-density lipoprotein cholesterol (HDLC), comparing it with the modified Lipid Research Clinics (LRC) procedure (heparin 183 kilounits/L, MnCI2 92 mmol/L) (U). Method I was insensitive to an HDLC concentration of 50 mg/L, but gave a linear dose-response curve between 130 and 1200 mg/L. Method II is sensitive to 50 mg/L and linear from 50-1200 mg/L. The within-plate CV for the Corning method varied from 26.2 % for an HDLC of 168 mg/L to 6.8 % for 580 mg/L. Within-day between-plate CV for the Corning method ranged from 22.1 % at 155 mg/Lto 8.0% at 651 mg/L, compared to3.0 and 0.8 % for the modified LRC procedure. Between-day CV for method I was 20, 12.6, 4.3, and 3.5 % for HDLC concentrations of 175, 435