Acutely Stimulates Skeletal Muscle 2-Deoxyglucose Uptake in Healthy Men

rodent muscle by exercise, metformin, 5-aminoimidazole-4-carboxamide 1--D-ribofuranoside (AICAR), and adi-ponectin increases glucose uptake. The aim of this study was to determine whether AICAR stimulates muscle glu-cose uptake in humans. We studied 29 healthy men (aged 26 8 years, BMI 25 4 kg/m2 [mean SD]). Rates of muscle 2-deoxyglucose (2DG) uptake were determined by measuring accumulation of total muscle 2DG (2DG and 2DG-6-phosphate) during a primed, continuous 2DG infu-sion. The effects of AICAR and exercise on muscle AMPK activity/phosphorylation and 2DG uptake were determined. Whole-body glucose disposal was compared before and during AICAR with the euglycemic-hyperinsulinemic clamp. Muscle 2DG uptake was linear over 9 h (R2 0.88 0.09). After 3 h, 2DG uptake increased 2.1 0.8- and 4.