Classification of the Efficacy of Herbal Medicine Alterations in Neuronal Hypoxia Models through Analysis of Gene Expression

Objectives: cDNA microarray is an effective method to snapshot gene expression. Functional clustering of gene expressions can identify herbal medicine mechanisms. Much microarray data is available for various herbal medicines. This study compares regulated genes with herbal medicines to evaluate the nature of the drugs. Methods: Published microarray data were collected. Total RNAs were prepared from dissociated hippocampal dissociate cultures which were given hypoxic shock in the presence of each herbal medicine. Up- or downregulated genes higher than Global M value 0.5 were selected, clustered in functional groups, and compared with various herbal treatments. Results: 1. Akt2 was upregulated by Acorus gramineus SOLAND, Arisaema amurense var. serratum NAKAI and Coptis chinensis FRANCH, and they belong to Araceae herb. 2. Nf-κb1, Cd5, Gnγ7 and Sgne1 were upregulated b