Adipose differentiation-related protein: a gonadotropin- and prostaglandinregulated protein in primate periovulatory follicles. Biol. Reprod. 72: 1305–1314. Downloaded from www.jlr.org at UIO Bibliotek for Medisin OG on May 25, 2007 Regulation of ADRP by

The midcycle LH surge stimulates a rise in follicular fluid prostaglandin E2 (PGE2), which is necessary for normal ovula-tion. To examine PGE2-regulated processes in primate follicles, monkey granulosa cells were cultured with hCG alone or with hCG and PGE2, and the resulting total RNA was subjected to microarray analysis. Twenty PGE2-regulated mRNAs were iden-tified, and we selected a lipid droplet protein, adipose differ-entiation-related protein (ADRP), for further study. To determine whether hCG and PGE2 regulate ADRP expression in vivo, mon-keys received gonadotropins to stimulate multiple follicular de-velopment. Human chorionic gonadotropin was then adminis-tered alone or with the PG synthesis inhibitor celecoxib, and follicular aspirates or whole ovaries were obtained at times that span the 40-h periovulatory interval. Administration of hCG in