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While membrane bioreactors (MBRs) feature significant advan-tages over conventional activated sludge (CAS) systems, and have gained increasing popularity for wastewater treatment and reclaim in the past decades, membrane fouling problem still remains the biggest barrier to the universal application of MBRs. Investigation of the membrane fouling mechanism should enable such fouling to be more predictable, and also facilitate to develop membrane ang et al., It generally believed that the deposition of a cake layer membrane surface is the major form of membrane fouling operation of MBRs (Wang et al., 2007; Lin et al., 2009, 2013; et al., 2012). Many researchers have been concentrating on inves-tigation of the foulants affecting cake layer formation. Biomass sludge was initially believed as the major factor affecting cake layer formation on membrane surface (Magara and Itoh, 1991)