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An especially interesting and important problem in the general area of interfacial phenomena concerns the behavior of monolayers composed of mixtures of amphiphilic molecules, some of which are soluble in an adjacent bulk phase and some of which are insoluble. Interaction of the soluble and insoluble species within monolayers at a vapor-liquid interface provides an excellent model to study various processes of biotechnological and physiological importance. Surfactants are used for a variety of reasons in bioprocesses: providing shear protection to cells in large suspended cultures; increasing the uptake and efficacy of drugs molecules; preventing electroporation of plasma membranes or even solubilizing the membranes for protein isolation. Successfully developing ways to predict the extent to which a soluble amphiphile present in a bulk phase will partition into a pre-formed insoluble monolayer, and how it subsequently affects the physical and chemical properties of the monolayer, will lead to a better understanding of these processes. Moreover, when used as a model membrane, it also provides a fundamental understanding of the nature of interactions between variou