Journal of Biotechnology, Computational Biology and Bionanotechnology RESEARCH PAPER Examination of ability to androgenesis of spring wheat genotypes resistant to Fusarium

Diseases caused by fungi from the genus Fusarium constitute a serious problem in spring wheat cultivation. Ear infestation leads reduced yield and plant contamination with mycotoxins. Therefore it is essential to introduce resistance genes to high-yielding cultivars. The generation of double haploid (DH) lines makes it possible to shorten the time required to select favourable genotypes. The aim of this study was to analyze the capacity of plants to regenerate in anther cultures and to generate DH of spring wheat genotypes that potentially constitute germplasm material for resistance breeding, directed against fungi from the genus Fusarium. The plant material comprised wheat cultivars with increased resistance to Fusarium: Sumai 3, Ning 8331, Norin 52, Frontana, as well as line 8475-59 and high-yielding Polish cultivars: Łagwa, Waluta and Zadra. Spikes were subjected to a thermal shock at 4EC. Anthers were placed on the C17 inducing medium. Two combinations of growth regula-tors, i.e. 2,4-D and kinetin as well as 2,4-D and dicamba were applied. A total of 19,200 anthers were used, resul-ting in a total of 440 calli from which 352 plants were obtained. Of this number 14 % were albino. The regeneration efficiency ranged from 0 % to 17.33 % depending on the analyzed genotype and the combination of growth regula-tors in the induction medium. The highest number of green plants and seeds were obtained from cv. Łagwa, Zadra and Sumai 3. Analyses of the regenerants, using a flow cytometer, demonstrated that depending on the genotype