Flow cytometric analysis of human bone marrow: I. Normal erythroid development

A panel of B lymphoid-reactive monoclonal antibodies was used to analyze the phenotypic changes that accompany B lymphocyte development in normal human bone marrow. The B lymphoid cells were identified using light scattering and the expression of CD1 9 on a flow cytometer. Quantita-tive three-color immunofluorescence was then used to correlate other cell surface antigens on these cells identi-fled as B lymphoid in normal marrow. CD1O and CD2O identified almost exclusive populations and provided a convenient means of discriminating between the less and more mature B lineage cells. The CD1O cells could be further subdivided using CD34. The population of CD19. CD1O. CD34 cells comprised only 0.6 % of marrow cells. but these contained the majority of terminal deoxynucleo-T HE BONE MARROW is the site of production an