and Oleg Mosin

The isotopic compositions of water its temperature and pH value were analyzed in experiments with modeling of primary hydrosphere. We performed experiments for the research of hot mineral and seawater from Bulgaria with IR-spectroscopy (differential non-equilibrium energy spectrum (DNES), Brucker Vertex Fourier-IR and Thermo Nicolet Avatar 360 Fourier-transform IR). It was shown that hot alkaline mineral water with temperature from +65 0 C to +95 0 C with pH = 9–11 is more suitable for the origination of life and living matter than other analyzed water samples. The pH value of seawater on the contrary is limited to the range of 7.5 to 8.4. In frames of the research the conditions imitating primary hydrosphere enriched with deuterium were also studied. The prokaryotic and eukaryotic cells of various microorganisms realizing methylotrophic, chemoheterotrophic, photoorganotrophic, and photosynthetic pathways of assimilation of carbon substrata were adapted to the maximal concentration of D2O, all biological material of which contained deuterium instead of hydrogen. Their studying would clear up the mechanism of function of deuterated macromolecules in conditions of hot D2O-solutions