Lactate production by swine adipocytes: effects of age, nutritional status, glucose concentration, and insulin

tion by swine adipocytes: effects of age, nutritional status, glucose concentration, and insulin. Am. J. Physiol. 272 (Endo-crinol. Metab. 35): E957-E966,1997.-To develop an alterna-tive model in which to study the relationship between adipose tissue lactate production, obesity, and non-insulin-dependent diabetes mellitus (NIDDM), we investigated lactate produc-tion by swine adipocytes. Subcutaneous adipocytes from fasted 3-wk-old, fasted ‘I-mo-old, and fed 7-mo-old Yucatan miniature swine were isolated and incubated with 0.2, 1, 5, 10, or 25 mM glucose t 1 mu/ml insulin. Total glucose metabolism (TGM) was estimated by product summation. Results showed that 1) TGM was threefold greater in cells from fasted 7-mo- vs. 3-wk-old swine (P < 0.05), 2) TGM was 2.7-fold greater in cells from fed ‘I-mo-old vs. fasted 7-mo-old swine (P < 0.05), 3) insulin failed to stimulate TGM i