1251-LabeledRadioimmunoassay Kits for Progesterone Evaluated for Use in an In Vitro Fertilization Program

Radioassay Systems Laboratories, RSL) for measurement of serum or plasma progesterone, to determine their suitability for use in in vitro fertilization programs. Both kits were suitably rapid for program requirements. Results by both were linear with concentration up to 60 nmol/L, and both had acceptable lower detection limits of 0.3 nmol/L. Kit-deter-mined progesterone concentrations (y) for 100 patients’ samples correlated well with results by our existing 3H radioimmunoassay method (y = 1.llx + 0.2, r = 0.965 for the DPC kit; y = 1.01 x + 1.4, r = 0.974 for the RSL kit). Mean analytical recovery for the RSL kit was 116%, that for the DPC kit, 202%. Within-batch precision, expressed as the mean CV for three concentrations of progesterone, was 6.5% for the RSL kit, and 16.4 % for the DPC kit; between-day CV was 8.1 % for the RSL kit, 17.7 % for the DPC kit. W