Antibody-dependent enhancement of dengue virus infeetion mediated by bispecifie antibodies against cell surface molecules other than Fc’y receptors

Antibody-dependent enhancement (ADE) of dengue virus infection occurs when eutralizing antibodies at sub-neutralizing concentrations or non-neutralizing antibodies form complexes with the virus. These virus-antibody complexes can then attach to a Fc7 receptor-bearing cell, via the Fc portion of the immuno-globulin, resulting in an increased number of infected cells. ADE may be responsible in part for the most severe clinical manifestations f dengue virus infection which include haemorrhage and shock. Three classes of human Fcy receptors exist, FcTRI, FcTRII and FcTRIII. In this study, we examined the effects of neuraminidase on ADE of dengue virus infection mediated by the low-affinity FcTRII. K562 cells, which express only FcTRII, treated with neuraminidase r sulted in augmentation o