Exogenous NO enhances hydrogen peroxide-mediated neutrophil adherence to cultured endothelial cells

enhances hydrogen peroxide-mediated neutrophil adherence to cultured endothelial cells. Am. J. Physiol. 274 (Lung Cell. Mol. Physiol. 18): L820–L826, 1998.—One important aspect of oxidant injury is the enhancement of neutrophil-endothe-lial adhesion by oxidants such as hydrogen peroxide. Recent studies suggest that nitric oxide (NO) can limit oxidant-mediated tissue injury, since inhibitors of endogenous NO synthesis often promote neutrophil-endothelial adhesion. However, less is known about the direct role of exogenous NO in modulating proadhesive effects of oxidants. The objective of this study was to examine how an NO donor modifies hydrogen peroxide-mediated adhesion of neutrophils to cul-tured endothelial cells. Human umbilical vein endothelial cell monolayers were exposed for 30 min to 0–0.1 mM hydrogen peroxide with or without the NO donor spermine-NONOat