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Nosocomial infections of multidrug-resistant Pseudomonas aeruginosa (MDRPA) are a growing concern in hospitalized patients in burn centers. The aim of this study was to investigate the flagellin profiling and antibiotic susceptibility of P. aeruginosa isolated from burn wound infections. During 8 month study, 73 clinically P. aeruginosa isolates collected from patients hospitalized in burn ward. P. aeruginosa isolates were identified using standard laboratory procedures. In vitro susceptibility of clinical isolates of P. aeruginosa to 6 antimicrobial agents were investigated by Clinical and Laboratory Standards Institute (CLSI 2012) Kirby-Bauer disk diffusion assay. The frequency of different type of flagellin was investigated by using specific primers and by PCR method. The resistance rates of our isolates to 6 tested antimicrobial agents were relatively high. Imipenem has good activity while tobramycin and ciprofloxacin do not have any effect on P. aeruginosa isolates. Of 73 isolates 59 (80.8%) were multidrug resistant. Twenty eight of 73 isolates were resistant to all antibiotics. Agarose gel electrophoresis of chromosomal DNA exhibited that 59 isolates (80.8%) of P. aeruginosa had type A flagellin while only 14 isolates (19.2%) had type b flagellin. Given the antibiotic failure treatment, it appears that alternative ways such as immunity to prevent of thes