Analysis of Tissue Responses to Glass Implants xB2O3 (1-X) Bi2O3 in Wistar Rats

Abstract. The aim of this study was to evaluate rat soft tissue reactions to glass xB2O3 (1-x) Bi2O3 by using a histological analysis. Glass (0.01 g) in glycerin (0.1 ml) was implanted into the cervical, interscapular and lumbar subcutaneous space of Wistar female rats (n=16). We used eight types of glasses, and injected eight lots of animals with gradient different Boron and Bismuth concentrations. Tissue specimens were analyzed at the biological time points of 7, 14 and 28 days by optical microscopy. A massive inflammatory reaction with an intense foreign body reaction and an increased invasion of fibrovascular tissue was observed by day 7-14 post-inoculation. The granulomatous foreign body reaction increased at 28 days. Cell population changed from mainly neutrophils, lymphocites, mast cells, eosinophils, and fibroblasts to mainly lymphohistiocytes, macrophages and epitheloid cells with increase neoangiogenesis after 28 days