Molecular Human Reproduction vol.5 no.6 pp. 495–497, 1999 Existence of human DAZLA protein in the cytoplasm of human oocytes

The human deleted in azoospermia-like autosomal (DAZLA) gene is thought to be a candidate gene for azoospermia. cDNA encoding the C-terminal 94 amino acid residues of human DAZLA was used to express a bacterial fusion protein which was then used to raise polyclonal antibodies in rabbits. Immunohistochemical analyses with the human DAZLA antiserum showed that the DAZLA protein is expressed at a cytoplasmic location in female germ cells. Available evidence suggests that the DAZLA gene is a participant in human oogenesis. Key words: DAZLA / immunohistochemistry/oocyte/oogenesi