volume 9 Number 121981 Nucleic Acids Research Sequence of a yeast DNA fragment containing a chromosomal replicator and a tRNA"^1 gene

The sequence of a- 1.9 kb Bam x Hind III fragment from yeast has been determined. This fragment is part of a " yeast 6.7 kb Hind III segment cloned into pBR322 (pY2O). The fragment carries a single gene for a glutamate tRNA which has no intron. Accord-ing to genetic analyses [l] this fragment also contains a yeast chromosomal replicator. We have analyzed the sequence for poten-tial open reading frames and for several structural features which are thought to be involved in the initiation of DNA repli-cation. Hybridization studies have revealed that portions of this sequence are repeated within the yeast genome