Effects of leukocyte capillary plugging in skeletal muscle ischemia-reperfusion injury

leukocyte capillary plugging in skeletal muscle ischemia-reperfusion injury. Am. J. PhysioZ. 271 (Heart Circ. Physiol. 40): H2653-H2660, 1996.-The purpose of this study was to examine the relationship between increased capillary net-work resistance due to leukocyte capillary plugging and tissue injury following ischemia-reperfusion (I/R>. After a 30-min complete ischemia in rat spinotrapezius muscle, the frequency and duration of leukocyte capillary plugging were measured throughout capillary networks and used to esti-mate the increase in network flow resistance for I/R alone, I/R with phalloidin (Pl), and I/R with both Pl and cytochalasin D. Propidium iodide (PI) was used to label nonviable muscle cell nuclei within the volume of tissue supplied by the capillary network, and counts were made before ischemia, immediately after reperfusion, and 1 h postreperfusion. For I/R alone an