Add to ORKGHypothesis: We hypothesised that primary bronchial epithelial cells (PBECs) from subjects with COPD respond differently to Pseudomonas aeruginosa lipopolysaccharide (PA LPS) after cigarette smoke extract (CSE) exposure than PBECs obtained from smokers without airflow obstruction (SWAO) and non-smokers (NS). Methods: PBECs from 16 COPD subjects, 10 SWAOand 9 NS were cultured at air-liquid interface. Cultures were incubated with CSE prior to stimulation with PA LPS. IL-6 and IL-8 were measured by ELISA and Toll-like receptor 4 expression by FACS. Activation of NF-κB was determined by western blotting and ELISA, and MAPK and caspase-3 activity by western blotting. Apoptosis was evaluated using Annexin-V staining and the terminal transferase-me...
Chronic obstructive pulmonary disease (COPD) is characterized by an abnormal innate immune response....
Smoking is a major risk factor for chronic obstructive pulmonary disease (COPD) and causes remodelin...
Lung airway epithelium is the first line of defense against inhaled particulates such as cigarette s...
For in vitro studies of airway pathophysiology, primary epithelial cells have many advantages over i...
In COPD inflammation driven by exposure to tobacco smoke results in impaired innate immunity in the ...
Chronic obstructive pulmonary disease (CCPD) is a disease primarily caused by cigarette smoking, whi...
SummaryChronic obstructive pulmonary disease (COPD) is a disease primarily caused by cigarette smoki...
<div><p>For in vitro studies of airway pathophysiology, primary epithelial cells have many advantage...
The disease is characterized by a progressive and largely irreversible decline in lung function, whi...
For in vitro studies of airway pathophysiology, primary epithelial cells have many advantages over i...
Extracellular Hsp70 (eHsp70) can activate immune cells via Toll-like receptors (TLR) 2 and 4, and in...
The human upper respiratory tract, including the nasopharynx, is colonized by a diverse array of mic...
Cigarette smoking is the major cause of chronic obstructive pulmonary disease (COPD) and lung cancer...
The integrity of the respiratory epithelium is crucial for airway homeostasis. Tobacco smoke exposur...
Cigarette smoke (CS) is the leading risk factor to develop COPD. Therefore, the pathologic effects o...
Chronic obstructive pulmonary disease (COPD) is characterized by an abnormal innate immune response....
Smoking is a major risk factor for chronic obstructive pulmonary disease (COPD) and causes remodelin...
Lung airway epithelium is the first line of defense against inhaled particulates such as cigarette s...
For in vitro studies of airway pathophysiology, primary epithelial cells have many advantages over i...
In COPD inflammation driven by exposure to tobacco smoke results in impaired innate immunity in the ...
Chronic obstructive pulmonary disease (CCPD) is a disease primarily caused by cigarette smoking, whi...
SummaryChronic obstructive pulmonary disease (COPD) is a disease primarily caused by cigarette smoki...
<div><p>For in vitro studies of airway pathophysiology, primary epithelial cells have many advantage...
The disease is characterized by a progressive and largely irreversible decline in lung function, whi...
For in vitro studies of airway pathophysiology, primary epithelial cells have many advantages over i...
Extracellular Hsp70 (eHsp70) can activate immune cells via Toll-like receptors (TLR) 2 and 4, and in...
The human upper respiratory tract, including the nasopharynx, is colonized by a diverse array of mic...
Cigarette smoking is the major cause of chronic obstructive pulmonary disease (COPD) and lung cancer...
The integrity of the respiratory epithelium is crucial for airway homeostasis. Tobacco smoke exposur...
Cigarette smoke (CS) is the leading risk factor to develop COPD. Therefore, the pathologic effects o...
Chronic obstructive pulmonary disease (COPD) is characterized by an abnormal innate immune response....
Smoking is a major risk factor for chronic obstructive pulmonary disease (COPD) and causes remodelin...
Lung airway epithelium is the first line of defense against inhaled particulates such as cigarette s...