Prentki P: Identification of protein binding sites in genomic DNA by two-dimensional gel electrophoresis

We describe a simple two-dimensional electrophoresis procedure to identify the recognition sites of DNA-blnding proteins within large DNA molecules. Using this approach, we have mapped E.coll IHF (Integration Host Factor) binding sites within phage Lambda (48 kb) and phage Mu (39 kb) DNA. We are also able to visualize IHF binding sites in E.coll chromosomal DNA (4,700 kb). We present an extension of this technique using direct amplification by PCR of the Isolated restriction fragments, which should permit the cloning of a collection of recognition sequences for DNA binding proteins in complex genomes