Effect of Two Methods of Sperm Cells Processing on Achieving Fecundity "In Vitro " in Sow Oocytes

Considerable progress has been made in the in vitro production of pig embryos using improved methods for in vitro maturation (IVM) and fertilization (IVF). Variation among boars, ejaculates and IVF protocols used in different laboratories appears to influence the incidence of polyspermy (Revel F. et al., 1995), after the first division that occurred in the zygote content within 48 hours of cultivation in the thermostat (Xu, X. et al., 1996a). To highlight the effect of the two methods of sperm processing in achieving fecundity "in vitro " on sow oocytes we have conducted two separate experiments: one consisted of placing together a group of cumulus-oocyte complex with processed sperm cells in Percoll concentration gradient, and the other consisted of a number of COC cultivated with processed sperm cells through swim-up procedure (J.J. Parrish et al., 1989) On the case of spermatozoids processing in Percoll concentration gradient, out if 92 COC used for in vitro fecundation, 13.04 % of it had the first cleavages. Only 6.90 % of the zygotes had the first cleavages out of 58 COC used on in vitro fecundation, there were used processed spermatozoids by the method „swim-up”. If we evaluate the results of in vitro fecundation, we see that from the total number o