Different modulation of steroidogenesis and prostaglandin production in frog ovary in vitro by ACE and ANG II

lation of steroidogenesis and prostaglandin production in frog ovary in vitro by ACE and ANG II. Am. J. Physiol. 273 (Regulatory Integrative Comp. Physiol. 42): R2089–R2096, 1997.—Our aim was to study the role of angiotensin-converting enzyme (ACE) and angiotensin II (ANG II) on ovarian steroidogenesis and prostaglandin production of am-phibian. Hormonal effects of ACE, ACE inhibitors, synthetic bullfrog angiotensin I (ANG I), and [Val5]ANG II were compared on frog ovaries of postreproductive and prereproduc-tive periods. Very high ACE activity was found in ovary of water frog (Rana esculenta) compared with other frog tissues, and this activity was inhibited by the typical ACE inhibitors, captopril and lisinopril. Frog ovary tissue in postreproductive and prereproductive periods was incubated in vitro in the presence of ACE (2.5 mU/ml), captopril (0.1 mM), lisinopri