In vitro and in vivo studies on the anticandidal activity of Carica papaya seed extract

Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT: Ten isolates, from 25 skin scraping of cutaneous infected site were diagnosed as Candida albicans. Infection with C. albicans was the highest among females than males with a rate of 80 % and 20 % respectively, and among patients with age 21-30 years(40%). In vitro antifungal susceptibility of ketoconazole, fluconazole, Nystatin, Amphotericin–B and Clotrimazole against clinical isolates of C. albicans indicate that the yeast isolates were susceptible (100%) to four antifungal, while 3 out of isolates were resistance to fluconazole with inhalation zone 20.4mm. The in vitro study using the seed extracts of Carica papaya at concentrations 10%, 25 % and 50 % inhibited the growth of C. albicans with rate 10mm, 9mm, 8mm, respectively compatible with control(0%) 15mm. In vivo antifungal activity studies on candidiasis in mice treated with the seed extract (2.5g/kg body weight), liver showed infiltration in lymphocyte and hydropic degeneration, while spleen and kidney showed in filtration of lymphocyte, compatible with negative control (not treatment) and positive control treated with ketoconazole