Genome Sequence of Microbacterium yannicii, a Bacterium Isolated from a Cystic Fibrosis Patient

positive bacterium isolated on Columbia colistin-nalidixic acid (CNA) agar with 5 % sheep blood from the sputum of a cystic fibrosis patient. The present study reports the draft genome of aMicrobacterium yannicii strain. Many new, emerging, and/or multidrug-resistant bacteriahave been recently reported in cystic fibrosis (CF) patients using different technologies, including new culture media and molecular methods (1, 2). We report the isolation ofMicrobacte-rium yannicii from a sputum sample from an adult CF patient. Microbacterium belongs to the familyMicrobacteriaceae (8), order Actinomycetales, classActinobacteria, which comprisesmainly aer-obic Gram-positive bacteria with high GC content and a pepti-doglycan defined by a B-type cross-linkage (9). Bacteria of the genusMicrobacterium are endophytic Gram-positive bacteria that usually live within plant hosts. From the mid-1990s, the presence of Microbacterium was recognized in human clinical specimens. The Gram-positive strain that was isolated on Columbia colistin-nalidixic acid (CNA) agar with 5 % sheep blood (bioMérieux) grew after 24 h of culture at 37°C with 5 % CO2. Although its pathogenicity in humans is unknown, this bacterium could be an emerging pathogen in the context of CF.Microbacterium yannicii has also been isolated from the roots of Arabidopsis thaliana (3). Here, we present the genome ofMicrobacterium yannicii. To date, only one Microbacterium sp. genome, of Microbacterium testa-ceum, has been reported (7). Genomic DNA from Microbacterium yannicii (strain PS01; CSUR reference no. P191) was fully sequenced by pyrosequencing using the genome sequencer Titanium (454 Life Sciences, Bran-ford, CT) (8). A library of paired-end fragments was created fol-lowing the manufacturer’s instructions (454 Life Sciences, Bran-ford, CT). This library was sequenced using the GS Titanium sequencer (454 Life Sciences, Branford, CT) (6). Reads originated from each strain were assembled into contig