Brief Communication: Growth Stimulation of Mouse Leukemia Cells by

SUMMARY-Cells of mouse lymphomas L1210 (V) and P1534 and myeloblastic tumor C1498 were highly dependent for growth in stationary culture on 2-mercaptoethanol, cysteamine, or certain other thiols and disulfides. Survival of normal splenic lymphocytes in vitro was enhanced by 2-mercaptoethanol.-J Natl Cancer Inst 49: 579-581, 1972. MANY leukemia and lymphoma cell lines show unusually strict growth requirements in vitro, which draw attention to anomalous metabolic processes and have practical implications in the design of agents for chemotherapy (1, 2). Hutchison et ai. (3) could obtain tissue culture lines of L 1210 (V) cells only by prolonged serial conditioning techniques in which cells were alternately transferred from culture to mice. Of 5 lines, only 1 retained lymphoblastic morphology. Using various standard media (4) (Eagle's basa