Gene transfer into human bone marrow hematopoietic cells mediated by adenovirus vectors. Blood 87:5032–5039

Human bone marrow mononuclear cells (BMMNCs) and en-riched CD34 positive (CD34') cells were transduced with ad-enovirus vectors encoding Escherichia coli P-galactosidase gene. Tranductions were carried out by 24-hour coincuba-tion with adenovirus vectors at different multiplicities of in-fections (moil. Efficacy of gene transfer into BM cells and expression of the gene product (ie, P-galactosidase) were studied using X-Gal histochemical staining and flow cytome-tric analysis. X-Gal staining demonstrated that the percent-age of positive cells at mois of 5 to 500 was 3.4 % to 34.5% for BMMNCs and 6.0 % to 20.0 % for enriched CD34+ cells. Similar results (1.5 % to 35.7 % for BMMNCs and 5.4 % to 24.2 % for enriched CD34+ cells) were obtained with flow cytometric analysis using fluorescein di-P-D-galactopyrano-side (FDG). Multicolor flow cytometry analysis, which in