lon 34,

do ing ic e for of simultaneously introduce two butanol synthetic operons into the chromosome and create two in-frame gene oci. uce butanol efficiently omic technology that Electronic Journal of Biotechnology 18 (2015) 138–142 Contents lists available at ScienceDirect Electronic Journalof n-butanol, the formation of this chemical would not be accomplished before the heterologous pathway is introduced into host cells; with enabled rapid genetic modification in host cells ' chromosome.expression plasmids, E. coli was able to accumulate significantly high 2. Materials and methodsrapid growth, and relatively rich knowledge on the regulation of its metabolic network [3]. Unlike Clostridia, E. coli is not a native produce