Linear mammalian artificial chromosomes (MACs) will require functional teiomeres, a centromere and the ability to replicate autonomously. We are investigating the possibility of developing MACs from yeast artificial chromosomes (YACs). Retrofitting vectors have been constructed to replace YAC teiomeres with cloned human telomeric DNA. A modified YAC was introduced into mammalian cells by spheroplast fusion and the frequency with which the retrofitted human telomeric DNA seeded the formation of a new telomere was determined by Bal31 digestion and cytogenetic analysis. The telomere adjacent to the selectable marker gene was functional in 5/46 clones (11 %) while the telomere 200 kb away at the other end of the YAC was functional In 1/46 clone...
Telomeres share some common features among eukaryotes, with few exceptions such as the fruit fly Dro...
When eukaryotes first evolved, a transition occurred from circular chromosomes to linear chromosomes...
We have shown functional complementation of a genetic deficiency in human cultured cells, using arti...
Linear mammalian artificial chromosomes (MACs) will require functional telomeres, a centromere and t...
Construction of a mammalian artificial chromosome (MAC) will develop our understanding of the requir...
A human artificial chromosome (HAC) vector was constructed from a 1-Mb yeast artificial chromosome (...
A yeast artificial chromosome (YAC) was constructed with a native autonomous replicating sequence (A...
Mammalian artificial chromosomes might prove to be useful vectors for somatic gene therapy. The func...
Yeast artificial chromosomes (YACs) have become essential research tools as they enable large fragme...
Telomeres are repetitive sections of DNA at the termini of linear chromosomes that protect the ends ...
Yeast artificial chromosomes (YACs) from the CEPH MegaYAC library (Paris, France) ranging in size fr...
A minilibrary of human DNA fragments was prepared in the vector YIP5 from a DNA preparation enriched...
<div><p>Telomeric and subtelomeric regions of human chromosomes largely consist of highly repetitive...
A modification vector has been constructed to facilitate the transfer of yeast artificial chromosome...
Telomeric and subtelomeric regions of human chromosomes largely consist of highly repetitive and red...
Telomeres share some common features among eukaryotes, with few exceptions such as the fruit fly Dro...
When eukaryotes first evolved, a transition occurred from circular chromosomes to linear chromosomes...
We have shown functional complementation of a genetic deficiency in human cultured cells, using arti...
Linear mammalian artificial chromosomes (MACs) will require functional telomeres, a centromere and t...
Construction of a mammalian artificial chromosome (MAC) will develop our understanding of the requir...
A human artificial chromosome (HAC) vector was constructed from a 1-Mb yeast artificial chromosome (...
A yeast artificial chromosome (YAC) was constructed with a native autonomous replicating sequence (A...
Mammalian artificial chromosomes might prove to be useful vectors for somatic gene therapy. The func...
Yeast artificial chromosomes (YACs) have become essential research tools as they enable large fragme...
Telomeres are repetitive sections of DNA at the termini of linear chromosomes that protect the ends ...
Yeast artificial chromosomes (YACs) from the CEPH MegaYAC library (Paris, France) ranging in size fr...
A minilibrary of human DNA fragments was prepared in the vector YIP5 from a DNA preparation enriched...
<div><p>Telomeric and subtelomeric regions of human chromosomes largely consist of highly repetitive...
A modification vector has been constructed to facilitate the transfer of yeast artificial chromosome...
Telomeric and subtelomeric regions of human chromosomes largely consist of highly repetitive and red...
Telomeres share some common features among eukaryotes, with few exceptions such as the fruit fly Dro...
When eukaryotes first evolved, a transition occurred from circular chromosomes to linear chromosomes...
We have shown functional complementation of a genetic deficiency in human cultured cells, using arti...