Sequence analysis of the human DNA flanking sites of human immunodeficiency virus type 1 integration

Human immunodeficiency virus type 1 (HIV-1) tagged with the Escherichia coli supF gene has been used to clone integrated HIV-1 proviruses. Sequence analysis of the 600 to 800 bp of human DNA adjacent to 29 clones revealed a propensity for HIV-1 to integrate near the Alu class of human repetitive elements. In the life cycle of human immunodeficiency virus type 1 (HIV-1), integration of the viral genome into the host DNA is required for the production of progeny virus (4). Much is known about the integration reaction, and numerous studies have described the general nature of retroviral integration sit