RP-HPLC ASSAY METHOD DEVELOPMENT FOR PARACETAMOL AND LORNOXICAM IN COMBINATION AND CHARACTERIZATION OF OXIDATIVE DEGRADATION PRODUCTS OF

A simple, specific, accurate and precise reverse phase high pressure liquid chromatographic method has been developed for the simultaneous determi-nation of paracetamol and lornoxicam from tablets and characterization of deg-radation products of lornoxicam by reverse phase C18 column (Inertsil ODS 3V C-18, 2504.6 mm, 5 μm). The sample was analyzed using buffer (0.02504 M):methanol in the ratio of 45:55, as a mobile phase at a flow rate of 1.5 mL/min and detection at 290 nm. The retention time for paracetamol and lorno-xicam was found to be 2.45 and 9.40 min, respectively. The method can be used for estimation of the combination of these drugs in tablets. The method was validated as per ICH guidelines. The linearity of the developed method was achieved in the range of 249.09 – 747.29 μg/mL (r2 = 0.9999) for paracet-amol and 4.0125–12.0375 μg/mL (r2 = 0.9999) for lornoxicam. Recoveries from tablets were between 98 and 102%. The method was validated with respect to linearity, accuracy, precision, robustness and forced degradation studies which further proved the stability-indicating power. During the forced degradation stu