Key words: wheat gliadins, aromatic amino acids, reversed-phase high-performance liquid chromatography (RP- HPLC), UV spectroscopy Studies were carried out to identify and detect potentially toxic proteins of wheat. The gliadin fractions were subjected to chromatographic and spectroscopic analyses to develop the relevant discriminants. The spectral analysis showed that these proteins differ considerably in their tryptophan-to-tyrosine molar ratios. A standard curve was used. The gliadin fractions were identified by comparing the calculated values of the tryptophan-to-tyrosine molar ratio with the values obtained based on the amino acid sequence of wheat gliadins in the Swiss-Prot/TrEMBL database. Based on the retention times and second deri...
<p>(A) Gliadin and gliadin-like extracts from four oat varieties and bread wheat used as reference. ...
The colored grain of wheat (Triticum aestivum L.) contains a large number of polyphenolic compounds ...
Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) instrumentation has been used...
Two gamma-gliadin fractions, designated gamma(III) and gamma(V), were purified from cv. Chinese Spri...
Three gliadin fractions designated yni-gliadin, yv-gliadin and o-gliadin were purified to homogeneit...
To separate gliadin from wheat flour, a novel and stability-indicating reversed-phase ultra performa...
The wheat storage proteins have the most important role in defining functional properties of flour. ...
A proteomics-based approach was used for characterizing wheat gliadins from an Italian common wheat ...
The present paper describes a method for the identification of intact high molecular weight glutenin...
The wheat proteins soluble in chloroform-methanol mixtures are associated with several kinds of food...
Gliadin RP-HPLC of hexaploid wheat is shown to give "fingerprint type" profiles which are suitable f...
The present paper describes a method for the identification of intact high molecular weight glutenin...
The wheat storage proteins, especially the high molecular weight glutenin subunits (HMW-GS), play im...
We have performed a detailed characterization and identification of wheat gluten proteins obtained f...
The central domain (CD) and terminal domains (TDs) of wheat gliadins contain high and low levels of ...
<p>(A) Gliadin and gliadin-like extracts from four oat varieties and bread wheat used as reference. ...
The colored grain of wheat (Triticum aestivum L.) contains a large number of polyphenolic compounds ...
Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) instrumentation has been used...
Two gamma-gliadin fractions, designated gamma(III) and gamma(V), were purified from cv. Chinese Spri...
Three gliadin fractions designated yni-gliadin, yv-gliadin and o-gliadin were purified to homogeneit...
To separate gliadin from wheat flour, a novel and stability-indicating reversed-phase ultra performa...
The wheat storage proteins have the most important role in defining functional properties of flour. ...
A proteomics-based approach was used for characterizing wheat gliadins from an Italian common wheat ...
The present paper describes a method for the identification of intact high molecular weight glutenin...
The wheat proteins soluble in chloroform-methanol mixtures are associated with several kinds of food...
Gliadin RP-HPLC of hexaploid wheat is shown to give "fingerprint type" profiles which are suitable f...
The present paper describes a method for the identification of intact high molecular weight glutenin...
The wheat storage proteins, especially the high molecular weight glutenin subunits (HMW-GS), play im...
We have performed a detailed characterization and identification of wheat gluten proteins obtained f...
The central domain (CD) and terminal domains (TDs) of wheat gliadins contain high and low levels of ...
<p>(A) Gliadin and gliadin-like extracts from four oat varieties and bread wheat used as reference. ...
The colored grain of wheat (Triticum aestivum L.) contains a large number of polyphenolic compounds ...
Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) instrumentation has been used...