Rapid,CompetitiveEnzymoimmunoassayforAlbuminin Urine

In this solid-phase competitive enzymoimmunoassayfor albuminin human urine,antiserumto humanserum albumin labeled with horseradish peroxidase (EC 1.11.1.7) is Incu-bated with solid-phase-boundhuman serum albuminin the presenceof sample or standard.Resultsobtained correlate well (r = 0.96) withthoseof an establishedfluoroimmunoas-say.The presentassaycoversthe range0.9 to200 mg/Land can be performedwithin1 h. These characteristics,together withthe simplicityof the assay protocol,make it veryuseful for monitoringlow concentrationsof albuminin unne. Detec-tion of such minimal albuminuriaallows initiationof therapy that may prevent development of clinical proteinuria and associateddiabetic nephropathy. AddItIonalKeyphrases:albuminuna. proteinuria diabetes meiitus nephropathy. screenin